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O.), Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada; and Department of Medical Genetics and Pediatrics, University of Calgary, Calgary, Alberta, Canada (A. Background—Fabry disease (FD) is an X-linked disorder of lysosomal metabolism affecting multiple organs with cardiac disease being the leading cause of death.Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a technique for the amplification of RNA.There is no need for expensive thermocycling equipment that is necessary for other methods like PCR, which makes RT-LAMP very cost effective.In contrast with conventional PCR and real-time PCR assays, this method is much more efficient while still obtaining a high level of precision.This process relies on thermal cycling, cycles of heating and cooling to facilitate the DNA replication.
Current imaging evaluations of the heart are suboptimal.
Within the last 10 years of its development, applications of the LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification have been widely used.
This method was then improved by taking it a step further and combining it with a reverse transcription phase to allow for the detection of RNA.
O.), Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada; and Department of Medical Genetics and Pediatrics, University of Calgary, Calgary, Alberta, Canada (A. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail.
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